Actin β Polyclonal Antibody Catalog No DM-Ab-03079Isotype IgG Reactivity Human;Mouse;Rat;Chicken;Globefish ;Bovine;Hamster;Pig;Ovine;Cat;Pig;Dog;Sheep Applications IF;WB;IHC;ELISA Gene Name ACTBProtein Name Actin cytoplasmic 1Immunogen Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80 Specificity Actin β Polyclonal Antibody detects endogenous levels of Actin β protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Dilution IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actinObserved Band 42kD Cell Pathway Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .Tissue Specificity B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular caraiwancq.com Function disease:Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) Background This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a matters needing attention Avoid repeated freezing and thawing! Usage suggestions This product can be used in immunological reaction related experiments. For Liu, Fengchao, et al. "Prolonged inhibition of class I PI3K promotes liver cancer stem cell expansion by augmenting SGK3/GSK-3β/β-catenin signalling." Journal of Experimental & Clinical Cancer Research 37.1 (2018): 122. Chen, Rui, et al. "Activation of CD137 Signaling Enhances Vascular Calcification through c-Jun (2018). [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing t lo yp ss e .s ,f o un f c c t e io ll n m :A o c t t il i i n ty s a a n re d h a i r g e h u lybi c q o u n it s o e u r s v l e y d e p xp ro re te s i s n e s d th i a n t a a ll re eu in k v a o ry lv o e t d ic in various cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the more information, please consult technical personnel. major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008], N-Terminal Kinase-Dependent Disruption of Autophagic Flux." Mediators of inflammation 2018aiwancq.com Xie, Changli, et al. "Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function." International journal of molecular medicine 38.1 (2016): 148-160. Yao, Chong, et al. "Crocin induces autophagic apoptosis in hepatocellular carcinoma by inhibiting Akt/mTOR activity." OncoTargets and therapy 11 (2018): 2017. L In i, tim Ha a i l m H in y g p ,e e rp t l a a l s . ia "C a o n ll d ag Im en pr E o x v t e er R n e a m l S o c d a e f l f i o n l g ds of M V it e ig in ate Grafts in a Rabbit Arteriovenous Graft Model." BioMed Research International 2017 (2017).
GAPDH Polyclonal Antibody Catalog No DM-Ab-03502Isotype IgG Reactivity Human;Mouse;Rat;Rabbit;Ch;Mk;sheep;X;Fish;Chicken;Guinea pig;Guinea pig;Duck Applications WB;IHC;IF Gene Name GAPDH Protein Name Glyceraldehyde-3-phosphate dehydrogenase Immunogen Recombinant Protein of GAPDH Specificity The antibody detects endogenous GAPDH protein. Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. Dilution WB: 1:5000 IHC: 1:200. IF 1:50-200 Concentration 1 mg/ml Purity ≥90%Storage Stability -20°C/1 year Synonyms GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH Observed Band 37kD Cell Pathway Cytoplasm, cytosol . Nucleus . Cytoplasm, perinuclear region . Membrane . Cytoplasm, cytoskeleton . Translocates to the nucleus following S-nitrosylation Tissue Specificity Astrocytoma,Brain,Cajal-Retzius cell,Colon adenocarcinoma,Epitheliu and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions (PubMed:12829261). .Function catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = glyceraldehyde-3-phosphate dehydrogenase fami Background glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens Thismatters needing attention Avoid repeated freezing and thawing! Usage suggestions This product can be used in immunological reaction related experiments. For more information, please consult technical personnel. Zhao, Shaorong et al. “Deciphering the performance of polo-like kinase 1 in triple-negative breast cancer progression according to the centromere protein U-phosphorylation pathway.” American journal of cancer research vol. 11,5 2142-2158. 15 May. 2021 3-phospho-D-glyceroyl phosphate + NADH.,function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.,online information:Glyceraldehyde 3-phosphate dehydrogenase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis.,sequence caution:Differs quite extensively.,similarity:Belongs to the gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferriZhao, TingTing, et al. "Tangshen formula modulates gut Microbiota and reduces gut-derived toxins in diabetic nephropathy rats." Biomedicine & Pharmacotherapy 129 (2020): 110325. Yi, Sheng, et al. "Peripheral nerve injury induces dynamic changes of tight junction components." Frontiers in physiology9 (2018): 1519. Chang, Ying, et al. "Lentivirus-mediated knockdown of astrocyte elevated gene-1 inhibits growth and induces a re p t o in p o to b s la is st t o h m ro a ug c h el M ls.A "P P K loS pa o th n w e a 1 y1 s .2 in (2 h 0 u 1 m 6 a ): n e0148763. Wang, Xiao, et al. "Knockdown of Phospholipase Cɛ (PLCɛ) Inhibits Cell Proliferation via Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN)/AKT Signaling Pathway in Human Prostate Cancer." Medical science monitor: international medical journal of experimental and clinical research 24 (2018): 254.
重组小鼠干扰素-γ(IFN-γ)简介： 小鼠IFN-γ（mIFN-γ）与人干扰素-γ（hIFN-γ）具有41%的序列同源性，是巨噬细胞激活因子，IFN-γ的活性形式是一个二聚体，是由两个反平行相互锁定的单体组成，启动IFN-γ/JAK/STAT通路。IFN-γ信号转导具有多种生物学功能，主要与宿主防御和免疫调节有关，包括抗病毒和抗菌防御、细胞凋亡、炎症反应、先天免疫和获得性免疫等。别名： Interferon gamma (IFN-γ), Mouse, Recombinant; Type II interferon; T cell interferon; MAF; IFNG; IFG; IFIyy易游体育 信息： 表达宿主：人 HEK293 细胞 来 源：小鼠 同 义 词：免疫干扰素,II 型干扰素,T 细胞干扰素,MAF 蛋白序列：DNA 序列编码小鼠干扰素-γ(NP_032363.1)表达带有 His 标签在 C 末端。 分 子 量：重组小鼠干扰素-γ蛋白包含 139 个氨基酸，预测理论分子量为 16.3kd。 纯 度：≥95%采用 SDS-PAGE 凝胶和高效液相色谱分析。 内 毒 素：＜0.1EU/ug 生物活性：活性测定是其抑制小鼠 WEHI-279 细胞增殖的能力。 预期 ED50是≤0.2ng/ml， 对应的特定活性为 ≥5x106IU/mg。 组 成：含有 6%甘露醇的无菌 PBS（PH 7.4）的冻干粉 性状（Character）：本品为白色疏松体 稳定性（Stability）：冻干制剂可在 4℃保存 24 个月，溶解后的液体可置于-20℃保存 6-12 个月，避免反复冻融。用途及描述 科研试剂，广泛应用于细胞生物学，药理学等科研方面，严禁用于人体。干扰素-γ 是由 CD4 和 CD8 T 淋巴细胞以及活化的 NK 细胞产生的一种不稳定的干扰素。 IFN-γ 受体存在于 大多数免疫细胞中， 它们通过增加Ⅰ 类γ 蛋白的表面表达来响应 IFN-γ 信号传导。 这将促进抗原呈递给 T- 助手(CD4+)细胞.干扰素-γ 信号在抗原提呈、 细胞和抗原识别 B、 T 淋巴细胞中， 调节抗原特异性免疫应答 期.此外， 干扰素-γ 刺激了许多淋巴细胞功能， 包括巨噬细胞、 NK 细胞和中性粒细胞的抗微生物和抗肿瘤 反应。 人干扰素-γ 具有物种特异性， 仅在人和灵长类细胞中具有生物活性。使用说明： 建议在打开前对该小瓶进行短暂离心，以将内容物离到底部。建议将冻干粉溶解于注射用水、灭菌的超纯水或无菌PBS中，浓度为100μg/ml。储备溶液应分装储存，以待进一步稀释至工作浓度。尽可能避免反复冻融。复溶后的细胞因子 短期保存请分装后存放于-20℃。【注意】 部分yy易游体育 我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。 本yy易游体育 仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 为了您的安全和健康，请穿实验服并戴一次性手套操作。
微量元素溶液SL-4是一种常用于科研领域的试剂，主要用于微生物生长所需的微量元素补充，可以配制多种培养基，如MSM培养基、脱氮硫微螺菌培养基、流行盐厌氧菌培养基等。以下是关于微量元素溶液SL-4的详细介绍：基本信息中文名称：微量元素溶液SL-4英文名称：Trace Elements Solution SL-4货号：DM-B2231用途：科研用途，培养基补充剂yy易游体育 特性无菌yy易游体育 ：本品经杀菌处理，为无菌yy易游体育 ，确保使用过程中的安全性。高倍母液：作为高倍浓缩液，使用时需按比例稀释至适当浓度。稳定性：在低温状态下可能析出，需妥善保存以避免影响使用效果。使用注意事项专用性：本品仅用于科研领域，不宜用于临床诊断或其他非科研用途。无菌操作：在使用过程中，需严格遵守无菌操作规程，避免微生物污染。个人防护：为了操作者的安全和健康，建议在操作过程中穿戴实验服和一次性手套。保存条件常温避光：需存放在常温且避光的环境中，以保持yy易游体育 的稳定性和有效性。保存期限：一般为1年（具体以yy易游体育 说明书为准），超过保存期限后需重新评估yy易游体育 是否适合继续使用。
MSM培养基，也被称为基础盐培养基（Minimal Salt Medium），是一种在微生物学和生物学研究中常用的培养基。它的主要特点是成分简单，仅包含微生物生长所必需的基本盐类和微量元素，而不含复杂的有机物质。这种培养基通常用于研究细菌对特定碳源的降解能力，或者用于分离具有降解能力的细菌。MSM培养基的组成MSM培养基的组成成分主要包括无机盐类，如磷酸盐、硫酸盐、氯化物等，以及必需的微量元素。具体的成分比例会根据不同的研究目的和微生物种类而有所调整。例如，一些MSM培养基配方中可能包含（每升）：Na2HPO4·2H2O：3.5gKH2PO4：1.0g(NH4)2SO4：0.5gMgCl2·6H2O：0.1g此外，还可能需要添加其他微量元素溶液，如SL-4，以提供微生物生长所需的微量营养。MSM培养基的配制方法一般来说，MSM培养基的配制方法包括以下几个步骤：称量：按照配方准确称取各种盐类和微量元素。溶解：将称取好的盐类和微量元素溶解于适量的蒸馏水中。调节pH值：使用酸或碱调节溶液的pH值至适宜范围，通常为7.0~7.5。定容：将溶液定容至所需体积。灭菌：将配制好的培养基进行高温高压灭菌处理，以杀死其中的杂菌。分装：将灭菌后的培养基分装至无菌容器中备用。MSM培养基的应用MSM培养基在微生物学和生物学研究中具有广泛的应用。它可以用于：研究微生物对特定碳源的利用能力。分离和筛选具有特定降解能力的微生物。评估微生物在不同环境条件下的生长性能。注意事项MSM培养基仅供科研使用，不得用于其他非科研用途。在使用过程中应严格遵守无菌操作规程以防止污染。配制好的培养基应妥善保存以避免变质或污染。
SDHB Polyclonal AntibodyCatalog No dm-04340 Isotype IgG Reactivity Human;Mouse;Rat;Fish Applications WB;IHC;IF;ELISA Gene Name SDHBProtein Name Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrialImmunogen The antiserum was produced against synthesized peptide derived from the Internal region of human SDHB. AA range:131-180 Specificity SDHB Polyclonal Antibody detects endogenous levels of SDHB protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000.. IF 1:50-200Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 yearSynonyms SDHB; SDH; SDH1; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; IpObserved Band 31kDCell Pathway Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.Tissue Specificity Brain,Fibroblast,Liver, Function catalytic activity:Succinate + ubiquinone = fumarate + ubiquinol.,cofactor:Binds 1 2Fe-2S cluster.,cofactor:Binds 1 3Fe-4S cluster.,cofactor:Binds 1 4Fe-4S cluster.,disease:Defects in SDHB are a cause of Cowden-like syndrome [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.,disease:Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant m
UQCRFS1 rabbit pAb货号 dm-18000 同位型 IgG 应用 WB 种属 Human;Mouse;Rat 靶点 UQCRFS1 基因名称 UQCRFS1 蛋白名称 Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC 1.10.2.2) (Complex III subunit 5) (Cytochrome b-c1 complex subunit 5) (Rieske iron-sulfur protein) (RISP) (Ubiquinol-cytochrome c reductase ir 免疫原 Synthesized peptide derived from human UQCRFS1特异性 This antibody detects endogenous levels of UQCRFS1 at Human, Mouse,Rat 组成 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. 来源 Polyclonal, Rabbit,IgG 稀释 WB 1:500-2000 纯化工艺 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. 分子量 30kD 功能 [Cytochrome b-c1 complex subunit Rieske, mitochondrial]: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation . The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried ac细胞定位 Mitochondrion inner membrane ; Single-pass membrane protein . 浓度 1 mg/ml 储存 -15°C to -25°C/1 year(Do not lower than -25°C) 有关注意事项 Avoid repeated freezing and thawing! 使用建议 This product can be used in immunological reaction related experiments. For more information, please consult technical personnel.
Catalog No dm-16383 Isotype IgG Reactivity Human;Mouse;Rat Applications WB;IHC;IF;ELISA Gene Name ATP5A1 Protein Name ATP synthase subunit alpha mitochondrial Immunogen The antiserum was produced against synthesized peptide derived from human ATP5A1. AA range:201-250 Specificity ATP5A Polyclonal Antibody detects endogenous levels of ATP5A protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 yearSynonyms ATP5A1; ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha; mitochondrial Observed Band 60kD Cell Pathway Mitochondrion . Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side . Cell membrane ; Peripheral membrane protein ; Extracellular side . Colocalizes with HRG on the cell surface of T-cells (PubMed:19285951). . Tissue Specificity Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord. Function function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.,
NDUB8 Polyclonal AntibodyCatalog No dm-05803 Isotype IgGReactivity Human;MouseApplications WB;ELISA Gene Name NDUFB8 Protein Name NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (Complex I-ASHI) (CI-ASHI) (NADH-ubiquinone oxidoreductase ASHI subunit)Immunogen Synthesized peptide derived from human protein . at AA range: 120-200Specificity NDUB8 Polyclonal Antibody detects endogenous levels of protein. Formulation Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. Source Polyclonal, Rabbit,IgG Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB 1:500-2000 ELISA 1:5000-20000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 year Synonyms Observed Band 20kD Cell Pathway Mitochondrion inner membrane ; Single-pass membrane protein ; Matrix side . Tissue Specificity Blood,Brain,Lung,Umbilical cord blood, Function function:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.,similarity:Belongs to the complex I NDUFB8 subunit family.,subunit:Complex I is composed of 45 different subunits., Background function:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.,similarity:Belongs to the complex I NDUFB8 subunit family.,subunit:Complex I is composed of 45 different subunits., matters needing attention Avoid repeated freezing and thawing!
人类风湿因子(RF)elisa试剂盒检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被活性氧簇（ROS）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成＊终的黄色。颜色的深浅和样品中的活性氧簇（ROS）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品活性。样品收集、处理及保存方法1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。 自备物品1.酶标仪（450nm）2.高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒温箱操作注意事项1.试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。2.实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。3.浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，＊终结果乘以5才是样本实际浓度。4.严格按照说明书中标明的时间、加液量及顺序进行温育操作。5.所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注：标准品（S0-S5）浓度依次为：0、30、60、120、240、480 U/ml试剂的准备 20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1.手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。2.自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。操作步骤1.从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。2.设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；3.样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。4.除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。5.弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。6.每孔加入底物A、B各50μL，37℃避光孵育15min。7.每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。试剂盒性能1.准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。2.灵敏度：＊低检测浓度小于1.0 U/ml。3.特异性：不与其它可溶性结构类似物交叉反应。4.重复性：板内、板间变异系数均小于15%。5.贮藏：2-8℃，避光防潮保存。6.有效期：6个月免责声明1.试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。2.严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。
31011502012822