大豆胰蛋白酶抑制因子(TI)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被大豆胰蛋白酶抑制因子(TI)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的大豆胰蛋白酶抑制因子(TI)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 样本不能含叠氮钠(NaN3),因为叠氮钠(NaN3)是辣根过氧化物酶(HRP)的抑制剂。2. 标本采集后尽早进行提取,提取按相关文献进行。3. 植物萃取液或其它相关样本:请1000 x g离心20分钟,取上清即可检测。4. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、50、100、200、400、800 ng/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于1.0 ng/mL。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Soybean trypsin inhibitor (TI) ELISA Kit instruction Intended useThis TI ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TI in the sample, this TI ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TI concentration. The concentration of TI in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storages1. Can’t detect the samples which contain NaN3, because NaN3 inhibits HRP activity of the horseradish peroxidase.2. Extract as soon as possible after Specimen collection, Extracted according to the relevant literature.Cell culture supernates and plant exact fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C) Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,50,100,200,400,800 ng/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 ng/ml6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
植物(Plant)烟草花叶病毒(TMV)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被烟草花叶病毒抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUTOFF值相比较,从而判定标本中烟草花叶病毒(TMV)的存在与否。样品收集、处理及保存方法1. 样本不能含叠氮钠(NaN3),因为叠氮钠(NaN3)是辣根过氧化物酶(HRP)的抑制剂。2. 标本采集后尽早进行提取,提取按相关文献进行。3. 植物取液或其它相关样本:请1000 x g离心20分钟,取上清即可检测。4. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本请按照操作步骤用样本稀释液适当稀释以达到试剂盒的的*佳检测效果。4.严格按照说明书中标明的时间、加液量及顺序进行温育操作。5.所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
斑马鱼(Zebrafish)低密度脂蛋白胆固醇(LDL-C)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被低密度脂蛋白胆固醇(LDL-C)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的低密度脂蛋白胆固醇(LDL-C)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、0.5、1、2、4、8 mmol/L试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于0.1 mmol/L。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)α半乳糖苷酶(α-Gal)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被α半乳糖甘酶(α-Gal)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的α半乳糖苷酶(α-Gal)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品活性。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗原-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、3、6、12、24、48 U/ml试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗原100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于1.0 U/ml。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human α-Galactosidase (α-Gal) ELISA Kit instruction Intended useThis α-Gal ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of α-Gal in the sample, this α-Gal ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus α-Gal concentration. The concentration of α-Gal in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,3,6,12,24,48 U/ml.Reagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 U/ml6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
人(Human) 诺如病毒(NV)ELISA检测试剂盒 本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被诺如病毒抗体的包被微孔中,依次加入标本、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUTOFF值相比较,从而判定标本中诺如病毒(NV)的存在与否。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本无需稀释,直接取50μL加样即可。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置阴、阳性对照孔和样本孔,阴、阳性对照孔中加入阴性对照、阳性对照各50μL;3. 待测样本孔先加待测样本10μL,再加样本稀释液40μL;4. 随后阴、阳性对照孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)沙门氏菌(Salmonella)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被沙门氏菌抗体的包被微孔中,依次加入标本、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUT OFF值相比较,从而判定标本中沙门氏菌(Salmonella)的存在与否。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本请按照操作步骤用样本稀释液适当稀释以达到试剂盒的的*佳检测效果。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置阴、阳性对照孔和样本孔,阴、阳性对照孔中加入阴性对照、阳性对照各50μL;3. 待测样本孔先加待测样本10μL,再加样本稀释液40μL;4. 随后阴、阳性对照孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)霍乱弧菌(Cholera)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被人霍乱弧菌抗体的包被微孔中,依次加入标本、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUTOFF值相比较,从而判定标本中人霍乱弧菌(Cholera)的存在与否。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本请按照操作步骤用样本稀释液适当稀释以达到试剂盒的的*佳检测效果。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置阴、阳性对照孔和样本孔,阴、阳性对照孔中加入阴性对照、阳性对照各50μL;3. 待测样本孔先加待测样本10μL,再加样本稀释液40μL;4. 随后阴、阳性对照孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)乙型流感病毒(Flu B)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被人乙型流感病毒(Flu B)抗体的包被微孔中,依次加入标本、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUTOFF值相比较,从而判定标本中人乙型流感病毒(Flu B)的存在与否。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本请按照操作步骤用样本稀释液适当稀释以达到试剂盒的的*佳检测效果。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置阴、阳性对照孔和样本孔,阴、阳性对照孔中加入阴性对照、阳性对照各50μL;3. 待测样本孔先加待测样本10μL,再加样本稀释液40μL;4. 随后阴、阳性对照孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)甲型流感病毒(Flu A)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗原一步夹心法酶联免疫吸附试验(ELISA)。往预先包被人甲型流感病毒(Flu A)抗体的包被微孔中,依次加入标本、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。用酶标仪在450nm波长下测定吸光度(OD 值),与CUTOFF值相比较,从而判定标本中人甲型流感病毒(Flu A)的存在与否。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 预处理后的样本请按照操作步骤用样本稀释液适当稀释以达到试剂盒的的*佳检测效果。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无阴性对照0.5mL 0.5mL 无阳性对照0.5mL 0.5mL 无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释样本稀释液6mL3mL无底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无 试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置阴、阳性对照孔和样本孔,阴、阳性对照孔中加入阴性对照、阳性对照各50μL;3. 待测样本孔先加待测样本10μL,再加样本稀释液40μL;4. 随后阴、阳性对照孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 1. 试验有效性:阳性对照孔OD值平均值≥1.00; 阴性对照孔OD值平均值≤0.15。2. 临界值(Cut off)计算:临界值=阴性对照孔平均值+0.153. 阴性判断:样品OD值<临界值(Cut off),样品为阴性4. 阳性判断:样品OD值>临界值(Cut off),样品为阳性试剂盒性能1. 准确性:阳性对照孔OD值平均值≥1.00;阴性对照孔OD值平均值≤0.15,说明试验结果有效。2. 特异性:不与其它可溶性结构类似物交叉反应。3. 重复性:板内、板间变异系数均小于15%。4. 贮藏:2-8℃,避光防潮保存。5. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)环磷酸鸟苷(cGMP)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被环磷酸鸟苷(cGMP)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的环磷酸鸟苷(cGMP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、2、4、8、16、32 nmol/L试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于0.1 nmol/L。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Cyclic guanosine monophosphate (cGMP) ELISA Kit instruction Intended useThis cGMP ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of cGMP in the sample, this cGMP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus cGMP concentration. The concentration of cGMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,2,4,8,16,32 nmol/LReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 0.1 nmol/L6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
小鼠(Mouse)乳酸脱氢酶A(LDHA)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被乳酸脱氢酶A(LDHA)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的乳酸脱氢酶A(LDHA)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品活性。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、0.5、1、2、4、8 IU/L试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。 4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于0.1 IU/L。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
小鼠乳酸脱氢酶A(LDH-A)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断 实验原理:本试剂盒应用双抗体夹心法测定标本中小鼠乳酸脱氢酶A(LDH-A)水平。用纯化的小鼠乳酸脱氢酶A(LDH-A)捕获抗体包被微孔板,制成固相抗体,往包被的微孔中依次加入小鼠乳酸脱氢酶A(LDH-A),再与HRP标记的检测抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的小鼠乳酸脱氢酶A(LDH-A)呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中小鼠乳酸脱氢酶A(LDH-A)含量。 试剂盒组成:试剂盒组成48孔配置96孔配置保存说明书1份1份封板膜2片2片密封袋1个1个酶标包被板1×481×962-8℃保存标准品0.3ml×6管0.3ml×6管2-8℃保存酶标试剂5 ml×1瓶10 ml×1瓶2-8℃保存样品稀释液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂A液3 ml×1瓶6 ml×1瓶2-8℃保存显色剂B液3 ml×1瓶6 ml×1瓶2-8℃保存终止液3 ml×1瓶6 ml×1瓶2-8℃保存20×浓缩洗涤液15ml×1瓶25ml×1瓶2-8℃保存注:标准品浓度依次为:2000、1000、 500、250、125、0 pg/mL. 样本处理及要求:1. 血清:室温血液自然凝固10-20分钟,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如出现沉淀,应再次离心。2. 血浆:应根据标本的要求选择EDTA或柠檬酸钠作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应该再次离心。3. 尿液:用无菌管收集,离心20分钟左右(2000-3000转/分)。仔细收集上清,保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照实行。4. 细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。5. 组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。6. 标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融.7. 不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。 操作步骤1. 标准品的加样:设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;。2. 加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品*终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。3. 加酶:每孔加入酶标试剂100μl,空白孔除外。4. 温育:用封板膜封板后置37℃温育60分钟。5. 配液:将20倍浓缩洗涤液用蒸馏水20倍稀释后备用。6. 洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。7. 显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟. 8. 终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。9. 测定:以空白孔调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。 注意事项:1. 试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存。样本在使用前也要在室温平衡60分钟。2. 浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果。3. 各步加样均应使用加样器,并经常校对其准确性,以避免试验误差。一次加样时间*好控制在5分钟内,如标本数量多,推荐使用排枪加样。4. 请每次测定的同时做标准曲线,*好做复孔。如标本中待测物质含量过高(样本OD值大于标准品孔第一孔的OD值),请先用样品稀释液稀释一定倍数(n倍)后再测定,计算时请*后乘以总稀释倍数(×n×5)。5. 封板膜只限一次性使用,以避免交叉污染。6. 底物请避光保存。7. 严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.8. 所有样品,洗涤液和各种废弃物都应按传染物处理。9. 本试剂不同批号组分不得混用。10. 如与英文说明书有异,以英文说明书为准。 计算:以标准物的浓度为横坐标,OD值为纵坐标,在坐标纸上绘出标准曲线,根据样品的OD 值由标准曲线查出相应的浓度;再乘以稀释倍数;或用标准物的浓度与OD值计算出标 准曲线的直线回归方程式,将样品的OD值代入方程式,计算出样品浓度,再乘以稀释 倍数,即为样品的实际浓度。 (此图仅供参考) 试剂盒性能:1. 样品线性回归与预期浓度相关系数R值为0.95以上。2. 批内变异系数与批间变异系数应分别小于10%和15% 。 检测范围: 62.5 pg/mL – 2000 pg/mL 灵敏度: *低检测浓度小于10 pg/mL 保存条件及有效期:1. 试剂盒保存: 2-8℃。2.有效期: 6个月Mouse Lactate Dehydrogenase AFOR RESEARCH USE ONLY Drug NamesGeneric Name:Mouse Lactate Dehydrogenase A(LDH-A)ELISA Kit.PurposeThis kit allows for the determination of LDH-A concentrations in Mouse serum, plasma, tissue homogenates and other biological fluids.Principle of the assayThe kit assay Mouse LDH-A level in the sample, use Purified Mouse LDH-A antibody to coat microtiter plate wells, make solid-phase antibody, then add LDH-A to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LDH-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.Materials provided with the kitMaterials provided with the kit48determinations96 determinationsStorageUser manual11Closure plate membrane22Sealed bags11Microelisa stripplate112-8℃Standard0.3ml×6 bottle0.3ml×6 bottle2-8℃HRP-Conjugate reagent5ml×1 bottle10ml×1 bottle2-8℃Sample diluent3ml×1 bottle6ml×1 bottle2-8℃Chromogen Solution A3ml×1 bottle6ml×1 bottle2-8℃Chromogen Solution B3ml×1 bottle6ml×1 bottle2-8℃Stop Solution3ml×1 bottle6ml×1 bottle2-8℃20×Wash solution15ml×1 bottle25ml×1 bottle2-8℃Note: Standard concentration was followed by: 2000、1000、500、250、125、0 pg/mL.Specimen requirements1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.4. cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS(PH7.2-7.4), Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.5. Tissue samples- After cutting samples, check the weight,add PBS(PH7.2-7.4), Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS(PH7.4), Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.Assay procedure1. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.2.add sample:Set blank wells separately (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.3.add enzyme:Add HRP-Conjugate reagent 100μl to each well, except blank well. 4.Incubate: After closing plate with Closure plate membrane ,incubate for 60 min at 37℃.5.Configurate liquid: 20-fold wash solution diluted 20-fold with distilled water and reserve.6.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.7.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃8.Stop the reaction:Add Stop Solution 50μl to each well, Stop the reaction(the blue color change to yellow color).9.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.Important notes1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.2. washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .4. if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.(×n×5).5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.6. The substrate evade the light preservation.7. Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.8. All samples, washing buffer and each kind of reject should according to infective material process.9. Do not mix reagents with those from other lots.CalculateTake the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density. This chartis for reference only Assay range62.5 pg/mL - 2000 pg/mLSensitivityThe minimum detectable dose is typically less than 10 pg/mLStorage and validity1.Storage: 2-8℃.2.validity: six months.
小鼠(Mouse)FMS样酪氨酸激酶3配体(Flt3L)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被FMS样酪氨酸激酶3配体(Flt3L)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的FMS样酪氨酸激酶3配体(Flt3L)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、5、10、20、40、80 ng/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于1.0 ng/mL。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。
人(Human)半乳糖凝集素3(Galectin-3)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被半乳糖凝集素3(Galectin-3)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的半乳糖凝集素3(Galectin-3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、4、8、16、32、64 pg/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。 4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于1.0 pg/mL。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Galectin-3 ELISA Kit instruction Intended useThis Galectin-3 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Galectin-3 in the sample, this Galectin-3 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Galectin-3 concentration. The concentration of Galectin-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,4,8,16,32,64 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
人(Human)白细胞介素1(IL-1)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被白细胞介素1(IL-1)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的白细胞介素1(IL-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、7.5、15、30、60、120 pg/mL试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL;空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于1.0 pg/mL。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interleukin 1 (IL-1) ELISA Kit instruction Intended useThis IL-1 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-1 in the sample, this IL-1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-1 concentration. The concentration of IL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,7.5,15,30,60,120 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
人(Human)尿素(Urea)ELISA检测试剂盒本试剂盒只能用于科学研究,不得用于医学诊断检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验(ELISA)。往预先包被尿素(Urea)抗体的包被微孔中,依次加入标本、标准品、HRP标记的检测抗体,经过温育并彻底洗涤。用底物TMB显色,TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成*终的黄色。颜色的深浅和样品中的尿素(Urea)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD 值),计算样品浓度。样品收集、处理及保存方法1. 血清:使用不含热原和内毒素的试管,操作过程中避免任何细胞刺激,收集血液后,3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆:EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液:3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆:将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存:如果样本收集后不及时检测,请按一次用量分装,冻存于-20℃,避免反复冻融,在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪(450nm)2. 高精度加样器及枪头:0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒温箱操作注意事项1. 试剂盒保存在2-8℃,使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶,这属于正常现象,水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中,密封(低温干燥)保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白;按照说明书操作时样本已经稀释5倍,*终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注:标准品(S0-S5)浓度依次为:0、0.5、1、2、4、8 mmol/L试剂的准备 20×洗涤缓冲液的稀释:蒸馏水按1:20稀释,即1份的20×洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板:甩尽孔内液体,每孔加满洗涤液,静置1min后甩尽孔内液体,在吸水纸上拍干,如此洗板5次。2. 自动洗板机:每孔注入洗液350μL,浸泡1min,洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条,剩余板条用自封袋密封放回4℃。2. 设置标准品孔和样本孔,标准品孔各加不同浓度的标准品50μL;3. 样本孔先加待测样本10μL,再加样本稀释液40μL(即样本稀释5倍);空白孔不加。4. 除空白孔外,标准品孔和样本孔中每孔加入辣根过氧化物酶(HRP)标记的检测抗体100μL,用封板膜封住反应孔,37℃水浴锅或恒温箱温育60min。5. 弃去液体,吸水纸上拍干,每孔加满洗涤液,静置1min,甩去洗涤液,吸水纸上拍干,如此重复洗板5次(也可用洗板机洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入终止液50μL,15min内,在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线:在Excel工作表中,以标准品浓度作横坐标,对应OD值作纵坐标,绘制出标准品线性回归曲线,按曲线方程计算各样本浓度值。 试剂盒性能1. 准确性:标准品线性回归与预期浓度相关系数R值,大于等于0.9900。2. 灵敏度:*低检测浓度小于0.1 mmol/L。3. 特异性:不与其它可溶性结构类似物交叉反应。4. 重复性:板内、板间变异系数均小于15%。5. 贮藏:2-8℃,避光防潮保存。6. 有效期:6个月免责声明1. 试剂盒仅供研究使用,不得用于临床实验或人体实验,否则所产生的一切后果,由实验者承担,本公司概不负责。2. 严格按照说明书操作,实验者违反说明书操作,后果由实验者承担。 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Urea ELISA Kit instruction Intended useThis Urea ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Urea in the sample, this Urea ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Urea concentration. The concentration of Urea in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 ℃ incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,0.5,1,2,4,8 mmol/LReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3. Add Sample: Add testing sample 10μl then add 40μl of Sample Diluent to testing sample well; Blank well doesn’t add anyting.4. Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 0.1 mmol/L6. Standard curve Storage: 2-8℃.validity: six months. FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
OmnimAbs是什么品牌创新生物技术品牌OmnimAbs是一个创新生物技术品牌,专注于提供高质量的单克隆抗体和抗原,服务于生命科学行业。OmnimAbs品牌自成立以来,已经积累了10年的经验,专注于供应抗体和免疫诊断yy易游体育 。其科学团队由单克隆抗体领域的专家组成,拥有在重组蛋白和荧光技术方面的专长。这些专长已经转化为yy易游体育 和创新,为心力衰竭、肾衰竭、外科感染和肿瘤标志物、HIV/趋化因子受体、信号转导和神经生物学等领域提供高质量的单克隆抗体和抗原。OmnimAbs不仅提供yy易游体育 ,还为工业和研究应用提供全面的支持数据服务和便捷的yy易游体育 搜索工具。此外,OmnimAbs还提供多种国际知名品牌的试剂与耗材,包括但不限于Ablab、Eurogentec、Chemicell、BrainBits、Emsdiasum、Dyesol、Bangs Laboratories、Macrocyclics、Flystuff、EYlabs、scientific Commodities、Xcessbio Biosciences、omicronbio、A-M systems、Glycotech、Diagnostic Automation、AK Scientific、emfret ANALYTICS、LaysanBio、Peptides International、ademtech、Skyspring nanomaterials、Biosearchtech、Hematologic Technologies等。这些品牌涵盖了广泛的领域,包括但不限于生物化学、分子生物学和细胞培养等。OmnimAbs还特别提到其进口胎牛血清yy易游体育 ,这是一种来源于美国的热灭活胎牛血清,适用于广泛的细胞培养应用,并经过无菌过滤处理,适用于昆虫细胞培养,促进稳定、健康的细胞生长。综上所述,OmnimAbs不仅是一个提供高质量生物技术yy易游体育 的品牌,还致力于满足生命科学领域的多样化需求,通过其专业的团队和全面的yy易游体育 支持,为科研人员和实验室提供便利和效率
PeproTech于1988年创立于美国新泽西州,经过26年的发展,已成为世界上*大的重组细胞因子和蛋白、相关抗体及ELISA试剂盒生产商之一。PeproTech公司已开发出2,000多种yy易游体育 ,其精良的技术保证了yy易游体育 的高质量、高可靠性和高稳定性。 Peprotech的yy易游体育 具有很高的性价比,在世界上众多高水平的实验室和研究机构中广泛使用。截至2013年底,已被13,000篇SCI文献引用。
赛默飞世尔科技公司(Thermo Fisher Scientific)是一家总部位于马萨诸塞州沃尔瑟姆的美国科技服务供应商,在1956年由数个公司合并而成,其主要客户类型包括医药和生物公司,医院和临床诊断实验室,大学、科研院所和政府机构,以及环境与工业过程控制装备制造商等,为其提供用于研究、分析、发现和诊断的分析仪器、设备、试剂和耗材、软件和服务。Thermo Scientific能够提供一整套包括高端分析仪器、实验室装备、软件、服务、耗材和试剂在内的实验室综合解决方案。
圣克鲁斯生物技术在生物医药研究市场的发展中处于****地位。在过去的30年中,本公司已把重点放在不断发展的研究单克隆抗体、生物化学、实验室器具和CRISPRyy易游体育 。
Tocris Bioscience是一家专卖生命科学研究chemicals, peptides and antibodies的知名品牌,其yy易游体育 也广为各大药厂、大学、研究机构,超过五万名科学研究者所采用。Tocris bioscience是位于英国布里斯托尔(Bristol)的高品质试剂提供商,共有2000多种yy易游体育 ,主要集中在神经科学和信号传导领域,yy易游体育 类型包括小分子、多肽、抗体、配体和化合物筛选文库等,主要yy易游体育 包括GPCR ligands,神经传递素,离子通道调控剂,信号通路抑制剂等,这些yy易游体育 被广泛选择性地用于阻断或激活生物学通路。
R&D细胞因子,R&D ELISA试剂盒,RD试剂yy易游体育 描述:我公司专业代理R&Dyy易游体育 ,yy易游体育 多样,涉及细胞因子,生物试剂,ELISA试剂盒。保证原装,价格,货期快,服务好!
QIAGEN,是全球**的样品制备和检测技术供应商。样品制备技术用来分离和处理从血液或组织等生物样品中的DNA,RNA和蛋白质,检测技术使这些分离的分子,在随后的分析中显现,如特定病毒的DNA。其使命是使客户在生命科学,应用测试,制药,分子诊断等方面实现卓越的成就和突破。
Invitrogen是全球**的生命技术公司,公司创立于1987年,总部位于美国加利福尼亚州卡尔斯巴德。主要为全球从事生命科学研究的学术、政府研究机构、医药和生物技术公司提供生物技术yy易游体育 和服务。Invitrogen将研发力量集中在包括功能基因组、蛋白组学、生物信息学和细胞生物学等生命科学研究领域内的突破性创新,向全球各主要实验室提供在研究中所需要的新技术、新yy易游体育 及服务。 Invitrogen旗下包括Invitrogen、Gibco、MolecularProbes、Dynal、Biosource、Caltag、Zymed、Panvera、InforMax、BioReliance等众多行业****。为分子生物、疾病研究,药物研发和生物制品生产等提供全面的技术和服务。
Cell Signaling Technology (CST) 是美国**的生物公司和细胞信号转导研究的**,提供特色的信号转导检测用抗体及磷酸化、乙酰化、甲基化、泛素化抗体、ELISA试剂盒、细胞因子、化学试剂、激酶等yy易游体育 。CST一家总部位于美国马萨诸塞州丹弗斯的私营公司,拥有专业的生产和研发队伍,致力于提供全球高品质创新型研究yy易游体育 ,以加速生物学认知
AbcamAbcam位于英国的剑桥科学园,成立于1998年,为生命科学研究提供严格验证的抗体,试剂,蛋白,试剂盒。超过11万种优质高效的蛋白研究工具,提供数据表、多项验证并附有客户评价,现货速达,帮您更快完成实验。
BioVision. Inc. 位于美国加州,是世界上****专著于凋亡和细胞信号研究的公司。其yy易游体育 质优、价廉、包装使用方便。BioVision致力于为研究者们提供*好、*新的研究工具而不断扩大yy易游体育 及服务质量。该公司提供用于检测早、中、晚期凋亡的试剂,可以检测凋亡发生的不同区域,包括:线粒体、胞浆、胞膜及胞核。
Cayman ChemicalCayman Chemical在位于密西根安娜堡(Ann Arbor, Michigan)66,000平方英尺的工厂生产并制作近6000多种不同的化合物、抗体及分析试剂盒。向全球科学工作者提供多研究领域的生化、免疫试剂和分析试剂盒,其yy易游体育 被广泛应用于肿瘤、氧化氮、神经学、凋亡、氧化性损伤、内分泌学等不同研究领域。Cayman Chemical可提供用于检测的特色试剂盒,也提供多种高质量试剂